Fitorremediation by Brassica spp. from using fitorregulators

The irrational application and inadequate management of fertilizers containing heavy metals, for example, cadmium (Cd), presents potential environmental damages due to its accumulation in soils. Recovery from this accumulation can be accomplished through phytoremediation, a sustainable and low cost alternative. The literature describes some plants with high phytoremediation potential, including plant species belonging to the botanical family Brassicaceae, which tolerated excess Cd, however the species may have different levels of phytotoxicity.One alternative to circumvent this problem is the use of plant regulators.BR (Brassinosteroid) belongs to a group of phytoregulators that have the potential to enhance phytoextraction and alleviate oxidative stress that occurs during metabolic activity.The plant enzymatic defensive system involves the action of enzymes such as superoxide dismutase (SOD; EC and peroxidases (POD; EC to prevent ROS accumulation and damage to vegetative growth.Thus, we intend to test the scientific hypothesis that the application of BR in species of Brassica genus under different Cd availability will make it impossible for plants to express their physiological growth potential.The experiment will be conducted in a triple factorial scheme with 4 replications and in three blocks, applying two dosages of 24-epibrassinolide (0 and 50 nM), grown in Petri dishes and polystyrene trays, containing 72 cells filled with vermiculite under conditions.controlled, and supply of three dosages of Cd (0, 0.5 and 1.0 mM) the evaluations will be the vegetative growth, germination rates and activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD).The means of treatments will be compared by Tukey test, when there is significant difference by analysis of variance (ANOVA), considering the significance level α = 0.05.

Brassica napus; Brassica rapa; brassinosteroid, antioxidant enzymes;
heavy metal.

Mayra Dhaiane Cabral da Silva

Daniel Baron

Iuri Emmanuel de Paula Ferreira

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