Effects of pulsed electromagnetic fields (100 Hz - 150 kHz) on seed germination of Allium cepa L.

Since the discovery of radio waves by German physicist Röentgen, in 1895, and the first radiographic impression, many studies were devoted to the interaction of these waves with living matter, giving rise to radiobiology. Throughout the twentieth century, models and animals are served as the basis for these studies, including the human being himself. However, low frequency electromagnetic waves (non-ionizing radiation) were considered harmless at the time. Theorists believed that direct effects of these radiations were unlikely. This thought was justified by the fact that non-ionizing radiation is absorbed by the surface portion of organisms, culminating in the propagation of weak electric currents with negligible thermal effects and, therefore, unable to break molecular bonds or cause structural damage to cells. Today, however, there is a large body of evidence on the influence of non-ionizing radiation on organisms such as oxidative stress, germination inhibition, change in growth rate and DNA damage. Unfortunately, the physical mechanisms responsible for these effects are not clearly and objectively elucidated. To understand the nature of the interaction between radio waves and living organisms, it is important to characterize the responses to a broad frequency spectrum. Only in this way can the process vectors that determine the biological pathway of action be revealed. From the exhibition of Allium cepa L. to pulsed electromagnetic fields at different frequencies, the research aims to detail the response pattern of the plant and investigate possible mechanisms of action, noting in response the following variables morphological analysis (normal seedlings, abnormal seedlings, average length of main roots and average diameter of cotyledon), germination analysis (rate of germinated seeds, germination speed index, average germination time, average germination speed), histological and cytological analyzes (micronucleus formation and mitotic index), and seedling growth analysis(height of shoot, stem length and dry matter mass). Addressing pulse-induced pulsed electromagnetic fields in the range 100 Hz - 150 kHz, the signal being generated by a PWM generator (pulse width modulation) and amplified by a circuit with associated Mosfet transistors.

pulsed electromagnetic fields, oxidative stress, germ inhibition, micronucleus:

Luis Rodrigo Vieira dos Santos

Iuri Emmanuel de Paula Ferreira

Daniel Baron

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