Brassinosteroid and application in phytormediation of cadmium (cd)

The intensification of anthropogenic activities related to industrialization and agriculture accentuated the environmental pollution by heavy metals such as cadmium (Cd), nonessential metal which, when absorbed by plants, can inhibit biochemical processes such as photosynthesis, nutrient absorption testing, increase the production of reactive oxygen species (ROS) and retard vegetative growth. Intense production of ROS stimulates the production of antioxidant enzymes such as catalase (CAT) that effectively acts on the oxidation of ROS. The literature reports phytoremediation as a sustainable alternative in which plants are used to absorb and accumulate pollutants from the environment. Many plant species are effective in phytoremediation of Cd, such as plants of the Brassica genus. The literature points out that, although these species are efficient in phytoremediation, the application of phytoregulators, such as brassinosteroids (BRs), can attenuate the effects of toxicity and increase the absorption of Cd by the plant. We intend to test the scientific hypothesis that the use of BRs in uncropped Brassica species under different Cd availability will not allow plants to express their physiological growth potential. Thus this study will investigate the effect of treatment of Brassica rapa L. and Brassica napus L. seeds, with two dosages of 24-epibrassinolide (0 and 50 nM), grown in Petri dishes and polystyrene trays under controlled conditions and supply of three doses of Cd (0, 0.5 and 1.0 mM) from the catalase enzyme (CAT) activity evaluation, of photosynthetic pigment content, ionic nutrient accumulation and germination indexes. An experiment will be conducted in a triple factorial scheme (2x2x3), with 4 replications, each composed of a 20 seed Petri dish and 72 cell polystyrene trays in three blocks. As the test media will be compared by Tukey test, when there is significant difference in ANOVA, considering the significance level α = 0.05.

antioxidant enzymes; brassica; Ionic accumulation; oxidative stress; phytoregulator.

Gabriel Antonio Bortoloti

Daniel Baron

Flávio Gabriel Bianchini

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